منابع مشابه
Partial Purification of Digitonin-Solubilized beta-Glucan Synthase from Red Beet Root.
An enriched glucan synthase fraction was obtained from red beet root microsomes by sequential extraction with the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and digitonin. The digitonin suspension was centrifuged on a glycerol gradient, where a glucan synthase peak with a specific activity of 30- to 40-fold over microsomes was recovered. Most protein contaminants were ...
متن کاملSugar beet (Beta vulgaris L.).
Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentia...
متن کاملImprovement of the Sugar Beet
SUGARCANE and sugar beet are the important sources of sugar. This foodstuff, wldcli today is one of the purest and cheapest in our dietary, two centuries ago was a luxury available only to the rich. The story of the rise of sugar production to meet an ever-increasing demand is a romance of agriculture and chemistry. The accomplishments of the plant breeder and the chemical engineer in making th...
متن کاملDiscovery of novel antifungal (1,3)-beta-D-glucan synthase inhibitors.
The increasing incidence of life-threatening fungal infections has driven the search for new, broad-spectrum fungicidal agents that can be used for treatment and prophylaxis in immunocompromised patients. Natural-product inhibitors of cell wall (1,3)-beta-D-glucan synthase such as lipopeptide pneumocandins and echinocandins as well as the glycolipid papulacandins have been evaluated as potentia...
متن کاملCloning and Molecular Characterization of β-1,3-Glucan Synthase from Sparassis crispa
A β-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble β-1,3-glucan (β-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a β-glucan synthase protein, c...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1987
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.84.3.565